2026“A Chemoselective and Stereodivergent Platform of Heme-Nitrene Transferases to Access Chiral Aryl-β-Amino Esters and An Investigation of the Sequence-Activity Landscape.” J.C. Reisenbauer, H.M. Carder, A. Mora, A. Jarmamillo, and F. H. Arnold. Angew. Chem. Int. Ed., February 17, 2026. https://doi.org/10.1002/anie.202525505.
“OXtal: An All-Atom Diffusion Model for Organic Crystal Structure Prediction.” E. Jin, A. C. Nica, M. Galkin, J. Rector-Brooks, K. L. K. Lee, S. Miret, F. H. Arnold, M. Bronstein, A. J. Bose, A. Tong, and C.-H. Liu. ICLR, 2026. https://doi.org/10.48550/arXiv.2512.06987. 
“Sequence-based generative AI design of versatile tryptophan synthases” T. Lambert, A. Tavakoli, G. Dharuman, J. Yang, V. Bhethanabotla, S. Kaur, M. Hill, A. Ramanathan, A. Anandkumar, F.H. Arnold. Nature Communications, Jan 14, 2026. https://doi.org/10.1038/s41467-026-68384-6 . 
“Controllable All-Atom Protein Generation with Latent Diffusion” A.X. Lu, J. Yang, Y. Long, T. Lambert, W. Yan, S.A. Robinson, K.K. Yang, V. Gligorjevic, K. Cho, R. Bonneau, N. C. Frey, F.H. Arnold, R. Abbeel. bioRxiv, Jan 7, 2026. https://doi.org/10.1101/2024.12.02.626353 . 
2025“A Biocatalytic Platform for the Synthesis of Organofunctional Silanes and Siloxanes.” Z. Zhang, T.J. Fulton, R.R. Maar, J.M. Roberts, D.E. Katsoulis, and F.H. Arnold. Angew. Chem. Int. Ed., Dec 26, 2025. https://doi.org/10.1002/anie.202522762.
“Steering generative models with experimental data for protein fitness optimization.” J. Yang, W. Chu, D. P. Khalil, R. Astudillo, B. J. Wittmann, F. H. Arnold, and Y. Yue. NeurIPS, Dec 2025. https://doi.org/10.48550/arXiv.2505.15093. 
“Evaluation of machine learning-assisted directed evolution across diverse combinatorial landscapes.” F.-Z. Li, J. Yang, K. E. Johnston, E. Cursoy, Y. Yue, and F. H. Arnold. Cell Systems, September 17, 2025. https://doi.org/10.1016/j.cels.2025.101387. 
“Illuminating the universe of enzyme catalysis in the era of artificial intelligence.” J. Yang, F.-Z. Li, Y. Long, and F. H. Arnold. Cell Systems, August 26, 2025. https://doi.org/10.1016/j.cels.2025.101372. 
“Enzymatic stereodivergent synthesis of azaspiro[2.y]alkanes.” J. L. Kennemur, Y. Long ,C. J. Ko, A. Das, and F. H. Arnold. J. Am. Chem. Soc., July 22, 2025. . 
“Substrate-aware zero-shot predictors for non-native enzyme activities.” F.-Z. Li, L. A. Radtke, K. E. Johnston, C.-H. Liu, Y. Yue, and F. H. Arnold. GEM Workshop, ICLR, 2025. (Presented at GEM workshop, ICLR 2025, Singapore). 
“Priestley Medal Address 2025: A random (mostly) uphill walk” F. H. Arnold. Chem. Eng. News. March 23, 2025. (Presented at ACS Spring 2025, San Diego, CA). 
“Active learning-assisted directed evolution” J. Yang, R. G. Lal, J. C. Bowden, R. Astudillo, M. A. Hameedi, S. Kaur, M. Hill, Y. Yue, and F. H. Arnold. Nat. Commun. January 16, 2025. 10.1038/s41467-025-55987-8. 
2024“LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning” Y. Long, A. Mora, F.-Z. Li, E. Gürsoy, K. E. Johnston, and F. H. Arnold. ACS Synth. Biol. December 24, 2024. 10.1021/acssynbio.4c00625.
“Catalyzing the future: recent advances in chemical synthesis using enzymes” J. C. Reisenbauer, K. M. Sicinski, and F. H. Arnold. Curr. Opin. Chem. Biol. October 5, 2024. 10.1016/j.cbpa.2024.102536. 
“Biocatalytic Synthesis of α-Amino Esters via Nitrene C–H Insertion” E. Alfonzo, D. Hanley, Z.-Q. Li, K. M. Sicinski, S. Gao, and F. H. Arnold. J. Am. Chem. Soc. September 27, 2024. 10.1021/jacs.4c09989. 
“A combinatorially complete epistatic fitness landscape in an enzyme active site” K. E. Johnston, P. J. Almhjell, E. J. Watkins-Dulaney, G. Liu, N. J. Porter, J. Yang, and F. H. Arnold. PNAS. July 29, 2024. 10.1073/pnas.2400439121. 
“Reaction Discovery Using Spectroscopic Insights from an Enzymatic C–H Amination Intermediate” A. Das, S. Gao, R. G. Lal, M. H. Hicks, P. H. Oyala, and F. H. Arnold. J. Am. Chem. Soc. July 22, 2024. 10.1021/jacs.4c05761. 
“Stereospecific Enzymatic Conversion of Boronic Acids to Amines” D. Hanley, Z.-Q. Li, S. Gao, S. C. Virgil, F. H. Arnold, and E. Alfonzo. J. Am. Chem. Soc. July 3, 2024. . 
“CARE: a Benchmark Suite for the Classification and Retrieval of Enzymes” J. Yang, A. Mora, S. Liu, B. J. Wittmann, A. Anandkumar, F. H. Arnold, and Y. Yue. aRxiv. June 21, 2024. 2406.15669. 
“The β-subunit of tryptophan synthase is a latent tyrosine synthase” P. J. Almhjell, K. E. Johnston, N. J. Porter, J. L. Kennemur, V. C. Bhethanabotla, J. Ducharme, and F. H. Arnold. Nat. Chem. Biol. May 14, 2024. 10.1038/s41589-024-01619-z. 
“Biocatalytic, enantioenriched primary amination of tertiary C–H bonds” R. Mao, S. Gao, Z.-Y. Qin, T. Rogge, S. J. Wu, Z.-Q. Li, A. Das, K. N. Houk, and F. H. Arnold. Nat. Catal. May 3, 2024. 10.1038/s41929-024-01149-w. 
“Opportunities and Challenges for Machine Learning-Assisted Enzyme Engineering” J. Yang, F.-Z. Li, and F. H. Arnold. ACS Cent. Sci. February 5, 2024. 10.1021/acscentsci.3c01275. 
“Breeding Better Enzymes” F. H. Arnold. Am. Sci. January – February 2024. https://www.americanscientist.org/article/breeding-better-enzymes. “Directed evolution of enzymatic silicon-carbon bond cleavage in siloxanes” N. S. Sarai, T. J. Fulton, R. L. O’Meara, K. E. Johnston, S. Brinkmann-Chen, R. R. Maar, R. E. Tecklenburg, J. M. Roberts, J. C. T. Reddel, D. E. Katsoulis, and F. H. Arnold. Science. January 25, 2024. 10.1126/science.adi5554. 
“Iron Heme Enzyme-Catalyzed Cyclopropanations with Diazirines as Carbene Precursors: Computational Explorations of Diazirine Activation and Cyclopropanation Mechanism” T. Rogge, Q. Zhou, N. J. Porter, F. H. Arnold, and K. N. Houk. J. Am. Chem. Soc. January 25, 2024. 10.1021/jacs.3c06030. 
“An engineered enzyme that enables the stereoconvergent alkylation of alkene mixtures” R. Mao, and F. H. Arnold. Nat. Synth. January 2, 2024. 10.1038/s44160-023-00446-9. “Enzymatic Assembly of Diverse Lactone Structures: An Intramolecular C–H Functionalization Strategy” D. J. Wackelin, R. Mao, K. M. Sicinski, Y. Zhao, A. Das, K. Chen, and F. H. Arnold. J. Am. Chem. Soc. January 2, 2024. 10.1021/jacs.3c11722. 
2023“Expanding Biocatalysis for Organosilane Functionalization: Enantioselective Nitrene Transfer to Benzylic Si–C–H Bonds” A. Das, Y. Long, R. R. Maar, J. M. Roberts, and F. H. Arnold. ACS Catal. December 16, 2023. 10.1021/acscatal.3c05370.
“Biocatalytic Construction of Chiral Pyrrolidines and Indolines via Intramolecular C(sp3)–H Amination” Z-Y. Qin, S. Gao, Y. Zou, Z. Liu, J. B. Wang, K. N. Houk, and F. H. Arnold. ACS Cent. Sci. December 14, 2023. 10.1021/acscentsci.3c00516. 
“Biocatalytic, stereoconvergent alkylation of (Z/E)-trisubstituted silyl enol ethers” R. Mao, D. M. Taylor, D. J. Wackelin, T. Rogge, S. J. Wu, K. M. Sicinski, K. N. Houk, and F. H. Arnold. Nat. Synth. November 2, 2023. 10.1038/s44160-023-00431-2. 
“Directed evolution for Si–C bond cleavage of volatile siloxanes in glass bioreactors” T. J. Fulton, N. S. Sarai, R. L. O’Meara, F. H. Arnold. Methods Enzymol. October 19, 2023. 10.1016/bs.mie.2023.09.014. “Directed evolution of P411 enzymes for amination of inert C–H bonds” A. Das, S. Gao, S. V. Athavale, E. Alfonzo, Y. Long, and F. H Arnold. Methods Enzymol. October 13, 2023. 10.1016/bs.mie.2023.09.009. 
“Enzymatic Nitrogen Incorporation Using Hydroxylamine” S. Gao, A. Das, E. Alfonzo, K. M. Sicinski, D. Rieger, and F. H. Arnold. J. Am. Chem. Soc. September 6, 2023. 10.1021/jacs.3c08053. 
“DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering” J. Yang, J. Ducharme, KE. Johnston, FZ. Li, Y. Yue, and F. H. Arnold. ACS Synth. Biol. July 31, 2023. 10.1021/acssynbio.3c00301. 
“Enantio- and Diastereoenriched Enzymatic Synthesis of 1,2,3-Polysubstituted Cyclopropanes from (Z/E)-Trisubstituted Enol Acetates” R. Mao, D. J. Wackelin, C. S. Jamieson, T. Rogge, S. Gao, A. Das, D. M. Taylor, K. N. Houk, and F. H. Arnold. J. Am. Chem. Soc. July 11, 2023. 10.1021/jacs.3c04870. 
“Reversing the Enantioselectivity of Enzymatic Carbene N−H Insertion Through Mechanism-Guided Protein Engineering” C. Calvó-Tusell, Z. Liu, K. Chen, F. H. Arnold, and M. Garcia-Borràs. Angew. Chem. Int. Ed. June 01, 2023. 10.1002/anie.202303879. 
“Machine Learning for Protein Engineering” K. E. Johnston, C. Fannjiang, B. J. Wittmann, B. L. Hie, K. K. Yang, Z. Wu. arXiv. May 26, 2023. arXiv:2305.16634v1 . “MicroED Structure of a Protoglobin Reactive Carbene Intermediate” E. Danelius, N. J. Porter, J. Unge, F. H. Arnold, and T. Gonen. J. Am. Chem. Soc. March 22, 2023. 10.1021/jacs.2c12004. 
“Chemodivergent C(sp3)–H and C(sp2)–H cyanomethylation using engineered carbene transferases” J. Zhang, A. O. Maggiolo, E. Alfonzo, R. Mao, N. J. Porter, N. M. Abney, and F. H. Arnold. Nat. Catal. January 19, 2023. 10.1038/s41929-022-00908-x. 
“Creating New Enzymes with Evolution” P. Almhjell and F. H. Arnold. Veloxsci. 2023. 10.60150/0XIM8S. 2022“Protoglobin-catalyzed formation of cis-trifluoromethyl-substituted cyclopropanes via carbene transfer.” L. Schaus, A. Das, A. M. Knight, G. Jimenez-Osés, K. N. Houk, M. Garcia-Borràs, F. H. Arnold, X. Huang. Angew. Chem. Int. Ed. December 19, 2022. 10.1016/10.1002/anie202208936.
“New additions to the arsenal of biocatalysts for noncanonical amino acid synthesis” E. Alfonzo, A. Das, and F. H. Arnold. Curr. Opin. Green Sustain. Chem. December 2022. 10.1016/j.cogsc.2022.100701. 
“Enzymatic Nitrogen Insertion into Unactivated C–H bonds” S. V. Athavale†, S. Gao†, A. Das†, S. C. Mallojjala, E. Alfonzo, Y. Long, J. S. Hirschi, and F. H. Arnold. J. Am. Chem. Soc. October 4, 2022. 10.1021/jacs.2c08285. Perspective by Derek Lowe: Zapping in Amine Groups. Synfacts highlight: Enzymatic Amination and Amidation of Unactivated Csp3–H Sites. 
“In vivo hypermutation and continuous evolution” R. S. Molina, G. Rix, A. A. Mengiste, B. Álvarez, D. Seo, H. Chen, J. E. Hurtado, Q. Zhang, J. D. García-García, Z. J. Heins, P. J. Almhjell, F. H. Arnold, A. S. Khalil, A. D. Hanson, J. E. Dueber, D. V. Schaffer, F. Chen, S. Kim, L. Á. Fernández, M. D. Shoulders & C. C. Liu. Nat. Rev. Methods Primers. May 19, 2022. 10.1038/s43586-022-00119-5. 
“Biocatalytic Carbene Transfer Using Diazirines” N. J. Porter, E. Danelius, T. Gonen, & F. H. Arnold J. Am. Chem. Soc. May 13, 2022. 10.1021/jacs.2c02723. 
“Biocatalytic One-Carbon Ring Expansion of Aziridines to Azetidines via a Highly Enantioselective [1,2]-Stevens Rearrangement” D. C. Miller, R. G. Lal, L. A. Marchetti & F. H. Arnold. J. Am. Chem. Soc. March 8, 2022. 10.1021/jacs.2c00251. 
“evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library” B. J. Wittmann, K. E. Johnston, P. J. Almhjell & F. H. Arnold. ACS Synth. Bio. February 17, 2022. 10.1021/acssynbio.1c00592. ACS Editors’ Choice Article. 
“Combining chemistry and protein engineering for new-to-nature biocatalysis” D. C. Miller, S. V. Athavale, & F. H. Arnold. Nat. Syn. January 12, 2022. 10.1038/s44160-021-00008-x. 
2021“An Enzymatic Platform for Primary Amination of 1-Aryl-2-alkyl Alkynes” Z. Liu, Z. Qin, L. Zhu, S. V. Athavale, A. Sengupta, Z. Jia, M. Garcia-Borràs, K. N. Houk, & F. H. Arnold J. Am. Chem. Soc. December 23, 2021. 10.1021/jacs.1c11340.
“Chapter 4: Engineering Enzymes for New-to-Nature Carbene Chemistry” S. Athavale, K. Chen, & F. H. Arnold. In Transition Metal-Catalyzed Carbene Transformations (eds J. Wang, C.-M. Che and M.P. Doyle). December 21, 2021. 10.1002/9783527829170.ch4. 
“Protein Sequence Design with Deep Generative Models” Z. Wu, K. E. Johnston, F. H. Arnold, & K. K. Yang. Curr. Opin. in Chem. Biol. December, 2021. 10.1016/j.cbpa.2021.04.004. 
“Dual-function enzyme catalysis for enantioselective carbon–nitrogen bond formation” Z. Liu, C. Calvó-Tusell, A. Z. Zhou, K. Chen, M. Garcia-Borràs & F. H. Arnold. Nat. Chem. October 18, 2021. 10.1038/s41557-021-00794-z. 
“Biocatalytic, Intermolecular C-H Bond Functionalization for the Synthesis of Enantioenriched Amides” S. Athavale, S. Gao, Z. Liu, S. C. Mallojjala, J. Hirschi, F. H. Arnold. Angew. Chemie. September 17, 2021. 10.1002/ange.202110873. 
“Informed training set design enables efficient machine learning-assisted directed protein evolution” B. J. Wittmann, Y. Yue, & F. H. Arnold. Cell Syst. August 19, 2021. 10.1016/j.cels.2021.07.008. 
“Advances in Machine Learning for Directed Evolution” B. J. Wittmann, K. E. Johnston & F. H. Arnold. Curr. Opin. in Struct. Biol. August, 2021. . 
“Asymmetric Alkylation of Ketones Catalyzed by Engineered TrpB” E. J. Watkins-Dulaney, N. P. Dunham, S. Straathof, S. Turi, & F. H. Arnold. Angew. Chemie. July 15, 2021. . 
“Biocatalytic Transformations of Silicon—the Other Group 14 Element” N. S. Sarai, B. J. Levin, J. M. Roberts, D. E. Katsoulis & F. H. Arnold. ACS Cent. Sci.. May 7, 2021. 10.1021/acscentsci.1c00182. 
“Origin and Control of Chemoselectivity in Cytochrome c Catalyzed Carbene Transfer into Si–H and N–H bonds” M. Garcia-Borràs, S. B. J. Kan, R. D. Lewis, A. Tang, G. Jimenez-Osés, F. H. Arnold, and K. N. Houk J. Am. Chem. Soc.. April 28, 2021. 10.1021/jacs.1c02146. 
“Navigating the Unnatural Reaction Space: Directed Evolution of Heme Proteins for Selective Carbene and Nitrene Transfer” Y. Yang & F. H. Arnold. Acc. Chem. Res.. Jan 25, 2021. 10.1021/acs.accounts.0c00591. 
2020“New-to-nature chemistry from old protein machinery: carbene and nitrene transferases” Z. Liu & F. H. Arnold. Curr. Op. in Biotech. Dec 25, 2020. 10.1016/j.copbio.2020.12.005.
“High Throughput Screening with SAMDI Mass Spectrometry for Directed Evolution” A. J. Pluchinsky, D. J. Wackelin, X. Huang, F. H. Arnold, & M. Mrksich. J.A.C.S. Nov 11, 2020. 10.1021/jacs.0c07828. 
“Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities” G. Rix, E. J. Watkins-Dulaney, P. J. Almhjell, C. E. Boville, F. H. Arnold, & C. C. Liu. Nat. Comm. Nov 6, 2020. 10.1038/s41467-020-19539-6. 
“Nature’s Machinery, Repurposed: Expanding the Repertoire of Iron-Dependent Oxygenases” N. P. Dunham & F. H. Arnold. ACS Catal. Sept 28, 2020. 10.1021/acscatal.0c03606. 
“Tryptophan Synthase: Biocatalyst Extraordinaire” E. J. Watkins-Dulaney, S. Straathof, & F. H. Arnold. ChemBioChem. July 16, 2020. 10.1002/cbic.202000379. 
“Signal Peptides Generated by Attention-Based Neural Networks” Z. Wu, K. Y. Yang, M. Liska, A. Lee, D. Wernick, D. Weiner, & F. H. Arnold. ACS Synth. Bio. July 10, 2020. 10.1021/acssynbio.0c00219. 
“Diversity-Oriented Enzymatic Synthesis of Cyclopropane Building Blocks” B. J. Wittmann, A. M. Knight, J. Hofstra, S. E. Reisman, S. B. J. Kan, & F. H. Arnold. ACS Catalysis. June 4, 2020. 10.1021/acscatal.0c01888. 
“Enzymatic Primary Amination of Benzylic and Allylic C(sp3)–H Bonds” Z. J. Jia, S. Gao & F. H. Arnold. J. Am. Chem. Soc. May 25, 2020. 10.1021/jacs.0c03428. 
“Enzymatic Lactone-Carbene C–H Insertion to Build Contiguous Chiral Centers” A. Z. Zhou, K. Chen & F. H. Arnold. ACS Catalysis. April 21, 2020. 10.1021/acscatal.0c01349. 
“Engineering Cytochrome P450s for Enantioselective Cyclopropenation of Internal Alkynes” K. Chen & F. H. Arnold. J. Am. Chem. Soc. March 29, 2020. 10.1021/jacs.0c01313. 
“Selective Enzymatic Oxidation of Silanes to Silanols” S. Bähr, S. Brinkmann-Chen, M. Garcia-Borràs, J. M. Roberts, D. E. Katsoulis, K. N. Houk, F. H. Arnold. Angewandte Chemie. March 24, 2020. 10.1002/anie.202002861. 
“Engineering new catalytic activities in enzymes” K. Chen, F. H. Arnold. Nature Catalysis. January 20, 2020. 10.1038/s41929-019-0385-5. 
2019“Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Ligands” N. W. Goldberg, A. M. Knight, R. K. Zhang, F. H. Arnold. J. Am. Chem. Soc. December 2, 2019. 10.1021/jacs.9b11608.
“Tailoring Tryptophan Synthase TrpB for Selective Quaternary Carbon Bond Formation” M. Dick, N. S. Sarai, M. W. Martynowycz, T. Gonen, F. H. Arnold. J. Am. Chem. Soc. November 20, 2019. 10.1021/jacs.9b09864.
“Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics” C. N. Bedbrook, K. K. Yang, J. E. Robinson, E. D. Mackey, V. Gradinaru, F. H. Arnold. Nature Methods. October 14, 2019. 10.1038/s41592-019-0583-8.
“An enzymatic platform for the asymmetric amination of primary, secondary and tertiary C(sp3)–H bonds” Y. Yang, I. Cho, X. Qi, P. Liu, F. H. Arnold. Nature Chemistry. October 14, 2019. 10.1038/s41557-019-0343-5.
“Direct Enzymatic Synthesis of a Deep-Blue Fluorescent Noncanonical Amino Acid from Azulene and Serine” E. J. Watkins, P. J. Almhjell, F. H. Arnold. ChemBioChem. September 12, 2019. 10.1002/cbic.201900497.
“Nitroalkanes as Versatile Nucleophiles for Enzymatic Synthesis of Noncanonical Amino Acids” D. K. Romney, N. S. Sarai, F. H. Arnold. ACS Catalysis. August 20, 2019. .
“Engineering Chemoselectivity in Hemoprotein-Catalyzed Indole Amidation” O. Brandenberg, D. Miller, U. Markel, A. O. Chaib, F. H. Arnold. ACS Catalysis. August 7, 2019. 10.1021/acscatal.9b02508.
“Machine-learning-guided directed evolution for protein engineering” K. Yang, Z. Wu, F. H. Arnold. Nature Methods. July 15, 2019. 10.1038/s41592-019-0496-6.
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“Enantiodivergent α-Amino C-H Fluoroalkylation Catalyzed by Engineered Cytochrome P450s” J. Zhang, X. Huang, R. K. Zhang, F. H. Arnold. J. Am. Chem. Soc. June 12, 2019. 10.1021/jacs.9b04344.
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“Directed Evolution of a Cytochrome P450 Carbene Transferase for Selective Functionalization of Cyclic Compounds” O. F. Brandenberg, K. Chen, F. H. Arnold. J. Am. Chem. Soc. May 9, 2019. 10.1021/jacs.9b02931.
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“Machine-Learning-Assisted Directed Protein Evolution with Combinatorial Libraries” Z. Wu, S. B. J. Kan, R. D. Lewis, B. J. Wittmann, F. H. Arnold. PNAS, April 12, 2019. 10.1073/pnas.1901979116. Corrected . Highlighted in Synfacts (doi: 10.1055/s-0039-1689894).
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“A Biocatalytic Platform for Synthesis of Chiral α-Trifluoromethylated Organoborons” X. Huang, M. Garcia-Borràs, K. Miao, S. B. J. Kan, A. Zutshi, K. N. Houk, F. H. Arnold. ACS Cent. Sci., February 1, 2019. 10.1021/acscentsci.8b00679. (ACS Central Science Highlight, Nature Catalysis Highlight)
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“Engineered Cytochrome c-Catalyzed Lactone-Carbene B–H Insertion” K. Chen, X. Huang, S.-Q. Zhang, A. Z. Zhou, S. B. J. Kan, X. Hong, F. H. Arnold. Synlett, January 15, 2019. 10.1055/s-0037-1611662. (Invited for Pearl Anniversary Issue of the 30 Years SYNLETT).
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“Enantioselective Aminohydroxylation of Styrenyl Olefins Catalyzed by an Engineered Hemoprotein” I. Cho, C. K. Prier, Z. Jia, R. K. Zhang, T. Görbe, F. H. Arnold. Angewandte Chemie, January 2 2019. 10.1002/anie.201812968.
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“Selective C–H bond functionalization with engineered heme proteins: new tools to generate complexity” R. K. Zhang, X. Huang, F. H. Arnold. Current Opinion in Chemical Biology, April 2019, 49:67–75. https://doi.org/10.1016/j.cbpa.2018.10.004.
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2018 |
“Enzymatic assembly of carbon–carbon bonds via iron-catalysed sp3 C–H functionalization” R. K. Zhang, K. Chen, X. Huang, L. Wohlschlager, H. Renata, F. H. Arnold. Nature. December 19, 2018. 10.1038/s41586-018-0808-5.
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“Engineering enzymes for noncanonical amino acid synthesis” P. J. Almhjell, C. E. Boville, F. H. Arnold. Chemical Society Reviews. December 21, 2018. 10.1039/C8CS00665B.
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“Alternate Heme Ligation Steers Activity and Selectivity in Engineered Cytochrome P450-Catalyzed Carbene-Transfer Reactions” K. Chen, S.-Q. Zhang, O. F. Brandenberg, X. Hong, F. H. Arnold. J. Am. Chem. Soc. October 29, 2018. 10.1021/jacs.8b09613.
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“Engineered biosynthesis of β‐alkyl tryptophan analogs” C. E. Boville, R. A. Scheele, P. Koch, S. Brinkmann-Chen, A. R. Buller, F. H. Arnold. Angewandte Chemie, September 14, 2018. 10.1002/anie.201807998
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“Chemistry Takes a Bath: Reactions in Aqueous Media” D. K. Romney, F. H. Arnold, B. H. Lipshutz, C. J. Li. JOC, July 20, 2018. 10.1021/acs.joc.8b01412.
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“Catalytic iron-carbene intermediate revealed in a cytochrome c carbene transferase” R. D. Lewis, M. Garcia-Borràs, M. J. Chalkley, A. R. Buller, K. N. Houk, S. B. J. Kan, F. H. Arnold. PNAS, June 26, 2018. 10.1073/pnas.1807027115.
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“Directed evolution mimics allosteric activation by stepwise tuning of the conformational ensemble” A. R. Buller, P. van Roye, J. K. B. Cahn, R. A. Scheele, M. Herger, F. H. Arnold. JACS, April 30, 2018. 10.1021/jacs.8b03490.
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“Improved Synthesis of 4-Cyanotryptophan and Other Tryptophan Analogues in Aqueous Solvent Using Variants of TrpB from Thermotoga maritima” C. E. Boville, D. K. Romney, P. J. Almhjell, M. Sieben, F. H. Arnold. JOC, April 13, 2018. 10.1021/acs.joc.8b00517.
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“Enzymatic construction of highly strained carbocycles” K. Chen, X. Huang, S. B. J. Kan, R. K. Zhang, F. H. Arnold. Science, April 6, 2018. .
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“Learned Protein Embeddings for Machine Learning” K. K. Yang, Z. Wu, C. N. Bedbrook, F. H. Arnold. Bioinformatics, March 23, 2018. .
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“Stereoselective Enzymatic Synthesis of Heteroatom-Substituted Cyclopropanes” O. F. Brandenberg, C. K. Prier, K. Chen, A. M. Knight, Z. Wu, F. H. Arnold. ACS Catalysis Editor’s Choice, February 24, 2018. 10.1021/acscatal.7b04423. Highlighted in Synfacts (doi: 10.1055/s-0037-1609665).
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“Diverse engineered heme proteins enable stereodivergent cyclopropanation of unactivated alkenes” A. M. Knight, S. B. J. Kan, R. D. Lewis, O. F. Brandenberg, K. Chen, F. H. Arnold. ACS Cent. Sci., February 21, 2018. 10.1021/acscentsci.7b00548. Highlighted in Synfacts (doi: 10.1055/s-0037-1610075).
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2017 |
“Genetically Programmed Chiral Organoborane Synthesis” S. B. J. Kan, X. Huang, Y. Gumulya, K. Chen, F. H. Arnold. Nature, November 29, 2017. doi:10.1038/nature24996.
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| “Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design” J. K. B. Cahn, S. Brinkmann-Chen, F. H. Arnold. In: Jensen M., Keasling J. (eds), Synthetic Metabolic Pathways. Methods in Molecular Biology Vol 1671, Humana Press, in press. |
“Directed Evolution: Bringing New Chemistry to Life” F. H. Arnold, Angewandte Chemie, November 28, 2017. https://doi.org/10.1002/anie.201708408. Also hosted here.
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| “Chapter 5. Directed evolution of artificial metalloenzymes: bridging synthetic chemistry and biology” R. K. Zhang, D. K. Romney, S. B. J. Kan, F. H. Arnold. In: Dieguez, M.; Bäckvall, J.-E.; Pamies, O. (eds), Artificial Metalloenzymes and MetalloDNAzymes in Catalysis. From Design to Applications, Wiley-VCH, in press. Available Here. |
“Machine Learning to Design Integral Membrane Channelrhodopsins for Efficient Eukaryotic Expression and Plasma Membrane Localization” C.N. Bedbrook, K.K. Yang, A. J. Rice, V. Gradinaru, F. H. Arnold, PLoS Computational Biology, October 23, 2017. doi: 10.1371/journal.pcbi.1005786.
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“Anti-Markovnikov Alkene Oxidation by Metal-oxo-mediated Enzyme Catalysis” S. C. Hammer, G. Kubik, E. Watkins, S. Huang, H. Minges, F. H. Arnold. Science, October 13, 2017. doi: 10.1126/science.aao1482. Selected by the Editorial Board of Synfacts (doi: 10.1055/s-0036-1592052).
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“Unlocking Reactivity of TrpB: A General Biocatalytic Platform for Synthesis of Tryptophan Analogues” D. K. Romney, J. Murciano-Calles, J. E. Wehrmuller, F. H. Arnold. Journal of the American Chemical Society, online publication July 14, 2017. doi:10.1021/jacs.7b05007.
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“Enantioselective Total Synthesis of Nigelladine A via Late-Stage C-H Oxidation Enabled by an Engineered P450 Enzyme” S. A. Loskot, D. K. Romney, F. H. Arnold, B. M. Stoltz. Journal of the American Chemical Society, online publication July 19, 2017. doi: 10.1021/jacs.7b05196.
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“Exploiting and Engineering Hemoproteins for Abiological Carbene and Nitrene Transfer Reactions” O. F. Brandenberg, R. Fasan, F. H. Arnold. Current Opinion in Biotechnology 47, 102-111 (2017). doi.org/10.1016/j.copbio.2017.06.005.
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“Design and Evolution of Enzymes for Non-natural Chemistry” S. C. Hammer, A. M. Knight, F. H. Arnold. Current Opinion in Green Sustainable Chemistry 7, 23-30 (2017). doi.org/10.1016/j.cogsc.2017.06.002.
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| “Enantioselective, Intermolecular Benzylic C-H Amination Catalysed by an Engineered Iron-Haem Enzyme” C. K. Prier, R. K. Zhang, A. R. Buller, S. Brinkmann-Chen, F. H. Arnold. Nature Chemistry 9, 629-634 (2017). doi:10.1038/NCHEM.278. Selected by the Editorial Board of Synfacts for its important insights as “Synfact of the month” (09/2017). |
“Chapter 1. Directed Evolution of an Allosteric Tryptophan Synthase to Create a Platform for Synthesis of Noncanonical Amino Acids” J. Murciano-Calles, A. R. Buller, F. H. Arnold. In M. Alcalde (ed.), Directed Enzyme Evolution: Advances and Applications, pp. 1-16 (2017). Springer International Publishing AG. doi: 10.1007/978-3-319-50413-1. |
| “Structure-Guided SCHEMA Recombination Generates Diverse Chimeric Channelrhodopsins” C. N. Bedbrook, A. J. Rice, K. K. Yang, X. Ding, S. Chen, E. M. LeProust, V. Gradinaru, F. H. Arnold. Proceedings of the National Academy of Sciences 114, E2624-E2633 (2017). doi/10.1073/pnas.170026911. |
“Directed Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a Synthetic Chromophore” L. Herwig, A. J. Rice, C. N. Bedbrook, R. K. Zhang, A. Lignell, J. K. B. Cahn, H. Renata, S. C. Dodani, I. Cho, L. Cai, V. Gradinaru, F. H. Arnold. Cell Chemical Biology 24, 415-425 (2017). http://dx.doi.org/10.1016/j.chembiol.2017.02.008.
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| “Engineering Green Biocatalysts for Chemical Reactions Not Known in Biology“ K. E. Hernandez. ACS Green Chemistry: The Nexus Blog (2017). |
2016 |
“Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity” A. R. Buller, P. van Roye, J. Murciano-Calles, F. H. Arnold. Biochemistry 55, 7043-7046 (2016). doi: 10.1021/acs.biochem.6b01127.
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“Directed Evolution of Cytochrome c for Carbon-Silicon Bond Formation: Bringing Silicon to Life” S. B. J. Kan, R. D. Lewis, K. Chen, F. H. Arnold. Science 354, 1048-1051 (2016). doi: 10.1126/science.aah6219.
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“Highly Stereoselective Biocatalytic Synthesis of Key Cyclopropane Intermediate to Ticagrelor” K. E. Hernandez, H. Renata, R. D. Lewis, S. B. J. Kan, C. Zhang, J. Forte, D. Rozzell, J. A. McIntosh, F. H. Arnold. ACS Catalysis 6, 7810-7813 (2016). doi: 10.1021/acscatal.6b02550.
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“A General Tool for Engineering the NAD/NADP Cofactor Preference of Oxidoreductases” J. Cahn, C. Werlang, A. Baumschlager, S. Brinkmann-Chen, S. Mayo, F. H. Arnold. ACS Synthetic Biology, online web publication September 30, 2016: doi: 10.1021/acssynbio.6b00188.
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“Identification of Mechanism-Based Inactivation in P450-Catalyzed Cyclopropanation Facilitates Engineering of Improved Enzymes” H. Renata, R. D. Lewis, M. J. Sweredoski, A. Moradian, S. Hess, Z. J. Wang, F. H. Arnold. Journal of the American Chemical Society 138, 12527-12533 (2016). doi:10.1021/jacs.6b06823
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“A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation” J. Murciano-Calles, D. K. Romney, S. Brinkmann-Chen, A. R. Buller, F. H. Arnold. Angewandte Chemie 55, 11577-11581 (2016). doi:10.1002/anie.201606242R1
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“Enhancement of Cellulosome-Mediated Deconstruction of Cellulose By Improving Enzyme Thermostability” S. Moraïs, J. Stern, A. Kahn, A. P Galanopoulou, S. Yoav, M. Shamshoum, M. A. Smith, D. G. Hatzinikolaou, F. H. Arnold, E. A. Bayer.Biotechnology for Biofuels 9, 164 (2016). doi:10.1186/s13068-016-0577-z
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“Synthesis of β-Branched Tryptophan Analogues Using an Engineered SubUnit of Tryptophan Synthase” M. Herger, P. van Roye, D. K. Romney, S. Brinkmann-Chen, A. R. Buller, F. H. Arnold. Journal of the American Chemical Society, 138, 8388-8391 (2016). doi:10.1021/jacs.6b04836
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| “The NAI Fellow Profile: An Interview with Dr. Frances Arnold” F. H. Arnold, K. A. Macuare Technology and Innovation 18, 79-82 (2016). |
“Discovery of a Regioselectivity Switch in Nitrating P450s Guided by MD Simulations and Markov Models” S. C. Dodani, G. Kiss, J. K. B. Cahn, Y. Su, V. S. Pande, F. H. Arnold. Nature Chemistry 8, 419-425 (2016). doi:10.1038/nchem.2474
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“Asymmetric Enzymatic Synthesis of Allylic Amines: A Sigmatropic Rearrangement Strategy” C. K. Prier, T. K. Hyster, C. C. Farwell, A. Huang, F. H. Arnold. Angewandte Chemie 55, 4711-4715 (2016). doi: 10.1002/anie.201601056
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| “Exploring the Mechanism Responsible for Cellulase Thermostability by Structure-Guided Recombination.” C. J. Chang, C. C. Lee, Y. T. Chan, D. L. Trudeau, M. H. Wu, C. H. Tsai, S. M. Yu, T. H. Ho, A. H. Wang, C. D. Hsiao, F. H. Arnold, Y. C. Chao.PLoS ONE 11(3), e0147485 (2016). doi: 10.1371/journal.pone.0147485 |
| “Mutations in Adenine Binding Pockets Enhance Catalytic Properties of NAD(P)H-Dependent Enzymes” J. K. B. Cahn, A. Baumschlager, S. Brinkmann-Chen, F. H. Arnold. Protein Engineering, Design and Selection 29, 31-38 (2016). doi: 10.1093/protein/gzv057 |
2015 |
| “Artificial Domain Duplication Replicates Evolutionary History of Ketol-Acid Reductoisomerases” J. K. B. Cahn, S. Brinkmann-Chen, A. R. Buller, F. H. Arnold. Protein Science 25, 1241-1248. doi: 10.1002/pro.2852 |
| “Directed Evolution of the Tryptophan Synthase β-Subunit for Stand-Alone Function Recapitulates Allosteric Activation“ A. R. Buller, S. Brinkmann-Chen, D. K. Romney, M. Herger, J. Murciano-Calles, F. H. Arnold. Proceedings of the National Academy of Sciences 112, 14599-14604 (2015). doi: 10.1073/pnas.1516401112 |
| “Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes to Create New Catalysts“ C. K. Prier, F. H. Arnold. Journal of the American Chemical Society 137, 13992-14006 (2015). doi: 10.1021/jacs.5b09348 |
| “Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450” J. A. McIntosh, T. Heel, A. R. Buller, L. Chio, F. H. Arnold. Journal of the American Chemical Society 137, 13861-13865 (2015) doi: 10.1021/jacs.5b07107 |
| “The Nature of Chemical Innovation: New Enzymes by Evolution” F. H. Arnold. Quarterly Reviews of Biophysics Discovery — The Nobel Workshop Issue 48, 404-410 (2015). doi:10.1017/S003358351500013X. |
| “Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo” C. N. Bedbrook, M. Kato, S. R. Kumar, A. Lakshmanan, R. D. Nath, F. Sun, P. W. Sternberg, F. H. Arnold, V. Gradinaru. Chemistry and Biology 22, 1108-1121 (2015). http://dx.doi:10.1016/j.chembiol.2015.06.020 |
| “Recent Advances in Engineering Microbial Rhodopsins for Optogenetics” R. S. McIsaac, C. N. Bedbrook, F. H. Arnold.Current Opinion in Structural Biology 33, 8-15 (August 2015). doi:10.1016/j.sbi.2015.05.001 |
| “Enantioselective Enzyme-Catalyzed Aziridination Enabled by Active-Site Evolution of a Cytochrome P450“ C. C. Farwell, R. K. Zhang, J. A. McIntosh, T. K. Hyster, F. H. Arnold. ACS Central Science 1, 89-93 (2015). doi: 10.1021/acscentsci.5b00056 |
| “Cofactor Specificity Motifs and the Induced Fit Mechanism in Class I Ketol-Acid Reductoisomerases“ J. K. Cahn, S. Brinkmann-Chen, T. Spatzal, J. A. Wiig, A. R. Buller, O. Einsle, Y. Hu, M. W. Ribbe, F. H. Arnold. Biochemical Journal 468 (part 3), 475-484 (2015). doi: 10.1042/BJ20150183 |
| “Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution“ H. Renata, Z. J. Wang, F. H. Arnold. Angewandte Chemie Int. Ed. 54, 3351-3367 (2015). doi: 10.1002/anie.201409470 |
| “P450 BM3-Axial Mutations: A Gateway to Non-Natural Reactivity“ T. K. Hyster, F. H. Arnold. Israel Journal of Chemistry 55, 14-20 (2015). doi: 10.1002/ijch.201400080 |
| “Directed Evolution of Gloebacter violaceus Rhodopsin Spectral Properties“ M. K. M. Engqvist, R. S. McIsaac, P. Dollinger, N. C. Flytzanis, M. Abrams, S. Schor, F. H. Arnold. Journal of Molecular Biology 427, 205-220 (2015). doi:10.1016/j.jmb.2014.06.015 |
2014 |
| “Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C-H Amination“ T. K. Hyster, C. C. Farwell, A. R. Buller, J. A. McIntosh, F. H. Arnold. Journal of the American Chemical Society 136, 15505-15508 (2014) doi: 10.1021/ja509308v |
| “Non-natural Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other Hemoproteins“ T. Heel, J. A. McIntosh, S. C. Dodani, J. T. Meyerowitz, F. H. Arnold. ChemBioChem 15, 2556-2562 (2014). doi: 10.1002/cbic.201402286 |
| “Structural, Functional, and Spectroscopic Characterization of the Substrate Scope of the Novel Nitrating Cytochrome P450 TxtE“ S. Dodani, J. K. B. Cahn, T. Heinisch, S. Brinkmann-Chen, J. A. McIntosh, F. H. Arnold. ChemBioChem 15, 2259-2267 (2014). doi: 10.1002/cbic.201402241 |
| “Uncovering Rare NADH-Preferring Ketol-Acid Reductoisomerases“ S. Brinkmann-Chen, J. K .B. Cahn, F. H. Arnold. Metabolic Engineering 26, 17-22 (2014). doi: 10.1016/j.ymben.2014.08.003 |
| “Directed Evolution of a Far-Red Fluorescent Rhodopsin“ R. S. McIsaac, M. K. M. Engqvist, T. Wannier, A. Z. Rosenthal, L. Herwig, N. C. Flytzanis, E. S. Imasheva, J. K. Lanyi, S. P. Balashov, V. Gradinaru, F. H. Arnold. Proceedings of the National Academy of Sciences 111, 13034-13039 (2014). doi: 10.1073/pnas.1413987111 |
| “Synthesis of Bioactive Protein Hydrogels by Genetically Encoded SpyTag-SpyCatcher Chemistry“ F. Sun, W.-B. Zhang, A. Mahdavi, F. H. Arnold, D. Tirrell. Proceedings of the National Academy of Sciences 111, 11269-11274 (2014). doi: 10.1073/pnas.1401291111 |
| “Archaerhodopsin Variants with Enhanced Voltage Sensitive Fluorescence in Mammalian and C. elegans Neurons“ N. C. Flytzanis, C. N. Bedbrook, H. Chiu, M. K. M. Engqvist, C. Xiao, K. Y. Chan, P. W. Sternberg, F. H. Arnold, V. Gradinaru. Nature Communications 5, 4894 (2014). doi: 10.1038/ncomms5894 |
| “P450-Catalyzed Asymmetric Cyclopropanation of Electron-Deficient Olefins Under Aerobic Conditions“ H. Renata, Z. J. Wang, R. Z. Kitto, F. H. Arnold. Catalysis Science & Technology 4, 3640-3643 (2014). doi:10.1039/C4CY00633J |
| “Engineered Thermostable Fungal Cellulases Exhibit Efficient Synergistic Cellulose Hydrolysis at Elevated Temperatures” D. L. Trudeau, T. M. Lee, F. H. Arnold. Biotechnology and Bioengineering 111, 2390-2397. doi: 10.1002/bit.25308 |
| “Enantioselective Imidation of Sulfides via Enzyme-Catalyzed Intermolecular Nitrogen-Atom Transfer“ C. C. Farwell, J. A. McIntosh, T. K. Hyster, Z. J. Wang, F. H. Arnold. Journal of the American Chemical Society 136, 8766-8771 (2014). doi: 10.1021/ja503593n |
| “Synthetic Biology: Engineering Explored” F. H. Arnold, J. T. Meyerowitz. Nature News & Views 509, 166-167 (May 8, 2014). |
| “Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables Formal Synthesis of Levomilnacipran” Z. J. Wang, H. Renata, N. E. Peck, C. C. Farwell, P. S. Coelho, F. H. Arnold. Angewandte Chemie Intl. Ed. 126, 6928-6931 (2014). doi:10.1002/ange.201402809R1 |
| “Isobutanol Production at Elevated Temperatures in Thermophilic Geobacillus thermoglucosidasius“ P. P. Lin, K. S. Rabe, J. L. Takasumi, M. Kadisch, F. H. Arnold, J. C. Liao. Metabolic Engineering 24, 1-8 (2014). http://dx.doi.org/10.1016/j.ymben.2014.03.006. . |
| “Expanding P450 Catalytic Reaction Space Through Evolution and Engineering” J. A. McIntosh, C. C. Farwell, F. H. Arnold. Current Opinion in Chemical Biology 19, 126-134 (2014). doi:10.1016/j.cbpa.2014.02.001 |
| “Chapter 9.15. Synthetic Biology Approaches for Organic Synthesis” P. S Coelho, F. H. Arnold, J. C. Lewis. In Gary A. Molander and Paul Knochel (eds.), Comprehensive Organic Synthesis, 2nd edition, Vol 9, pp. 390-420 (2014). Oxford: Elsevier. doi.org/10.1016/B978-0-08-097742-3.00931-9 |
| “Cytochrome P450-Catalyzed Insertion of Carbenoids into N-H Bonds” Z. J. Wang, N. E. Peck, H. Renata, F. Arnold. Chemical Sciences 5, 598-601 (2014). doi:10.1039/c3sc52535j |
| F. H. Arnold. In K. Wüthrich, I.A. Wilson, D. Hilvert, D.W. Wolan, A. De Wit (eds.) New Chemistry and New Opportunities from the Expanding Protein Universe. 23rd International Solvay Conference on Chemistry, pp. 84-89 (2014), World Scientific Publishing Co. Pte. Ltd. ISBN 978-9814603829. |
| “Photo-Limited and Electron Transfer Reactivity of Ruthenium-Labeled Cytochrome P450” M. E. Ener, P. S. Coelho, Z. J. Wang, F. H. Arnold, J. R. Winkler, H. B. Gray. Journal of Biological Inorganic Chemistry 19 (S1), S219 (2014). ISSN 0949-8257. |
2013 |
| “Innovation By Homologous Recombination” D. L. Trudeau, M. A. Smith, F. H. Arnold. Current Opinion in Chemical Biology17, 902-909 (2013). http://dx.doi.org/10.1016/j.cbpa.2013.10.007 |
| “Controlling Macromolecular Topology with Genetically Encoded SpyTag-SpyCatcher Chemistry” W.-B. Zhang, F. Sun, D. A. Tirrell, F. H. Arnold. Journal of the American Chemical Society 135, 13988-13997 (2013). doi:10.1021/ja4076452 |
| “Enantioselective Intramolecular C-H Amination Catalyzed by Engineered Cytochrome P450 Enzymes in vitro and in vivo“ J. A. McIntosh, P. S. Coelho, C. C. Farwell, Z. J. Wang, J. C. Lewis, T. R. Brown, F. H. Arnold. Angewandte Chemie International Edition 52, 9309-9312 (2013). doi:10.1002/anie.201304401 |
| “A Serine-Substituted P450 Catalyzes Highly Efficient Carbene Transfer to Olefins in vivo“ P. S. Coelho, Z. J. Wang, M. E. Ener, S. A. Baril, A. Kannan, F. H. Arnold, E. M. Brustad. Nature Chemical Biology 9, 485- 487 (2013). doi:10.1038/nchembio.1278 |
| “Hypocrea jecorina Cellobiohydrolase I Stabilizing Mutations Identified Using Noncontiguous Recombination” M. A. Smith, C. N. Bedbrook, T. Wu, F. H. Arnold. ACS Synthetic Biology 2, 690-696 (2013). doi: 10.1021/sb400010m |
| “General Approach to Reversing Ketol-Acid Reductoisomerase Cofactor Dependence from NADPH to NADH” S. Brinkmann-Chen, T. Flock, J. K. B. Cahn, C. D. Snow, E. M. Brustad, J. A. McIntosh, P. Meinhold, L. Zhang, F. H. Arnold. Proceedings of the National Academy of Sciences 110, 10946-10951 (2013). doi:10.1073/pnas.1306073110 |
| “Role of Cysteine Residues in Thermal Inactivation of Fungal Cel6A Cellobiohydrolases” I. Wu, T. Heel, F. H. Arnold. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 1834, 1539-1544 (2013). doi: 10.1016/j.bbapap.2013.05.003 |
| “Chapter 14. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI” P. A. Romero, M. G. Shapiro, F. H. Arnold, A. Jasanoff. In M. R. Banghart (ed.) Chemical Neurobiology: Methods and Protocols – Methods in Molecular Biology, Vol. 995, pp. 193-205 (2013). Springer Science-Business Media, New York. doi: 10.1007/978-1-62703-345-9_14 |
| “High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase” R. Lauchli, K. S. Rabe, K. Z. Kalbarczyk, A. Tata, T. Heel, R. Z. Kitto, F. H. Arnold. Angewandte Chemie, Intl. Ed. 52, 5571-5574 (2013). doi: 10.1002/anie.201301362 |
| “Chapter Sixteen – Efficient Sampling of SCHEMA Chimera Families to Identify Useful Sequence Elements” P. Heinzelman, P. A. Romero, F. H. Arnold. In A. E. Keating (ed.) Methods in Enzymology, Vol. 523, pp. 351-368 (2013). Burlington: Academic Press. ISBN: 978-0-12-394292-0. |
| “Engineered Thermostable Fungal Cel6A and Cel7A Cellobiohydrolases Hydrolyze Cellulose Efficiently at Elevated Temperatures“ I. Wu, F. H. Arnold. Biotechnology & Bioengineering, 110, 1874-1883 (2013). doi: 10.1002/bit.24864 |
| M. A. Smith, P. A. Romero, T. Wu, E. M. Brustad, F. H. Arnold. Protein Science 22, 231-238 (2013). doi: 10.1002/pro.2202 |
| “Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes P. S. Coelho, E. M. Brustad, A. Kannan, F. H. Arnold. Science 339, 307-310 (2013). doi: 10.1126/science.1231434 . |
| P. A. Romero, A. Krause, F. H. Arnold. Proceedings of the National Academy of Sciences 110(3), E193-E201 (2013). www.pnas.org/cgi/doi/10.1073/pnas.1215251110 |
2012 |
| “A Diverse Set of Family 48 Bacterial Cellulases Created By Structure-Guided Recombination” M. A. Smith, A. Rentmeister, C. D. Snow, T. Wu, M. F. Farrow, F. Mingardon, F. H. Arnold. FEBS Journal 279, 4453-4465 (2012). doi: 10.1111/febs.12032 |
| P. A. Romero, F. H. Arnold. PLoS Computational Biology 8(10), e1002713 (2012). doi:10.1371/journal.pcbi.1002713 |
| R. S. Komor, P. A. Romero, C. B. Xie, F. H. Arnold. Protein Engineering, Design and Selection, 25, 827-833 (2012). doi: 10.1093/protein/gzs058 |
| “Structure-Guided Engineering of Lactococcus lactis Alcohol Dehydrogenase LlAdhA for Improved Conversion of Isobutyraldehyde to Isobutanol” X. Liu, S. Bastian, C. D. Snow, E. M. Brustad, T. E. Saleski, J-H. Xu, P. Meinhold, F. H. Arnold. Journal of Biotechnology 164, 188-195 (2012). doi: 10.1016/j.jbiotec.2012.08.008 |
| E. M. Brustad, V. S. Lelyveld, C. D. Snow, N. Crook, S. T. Jung, F. M. Martinez, T. J. Scholl, A. Jasanoff, F. H. Arnold. Journal of Molecular Biology 422, 245-262 (2012). doi: 10.1016/j.jmb.2012.05.029. |
| P. A. Romero, E. Stone, C. Lamb, L. Chantranupong, A. Krause, A. Miklos, R. A. Hughes, B. Fechtel, A. D. Ellington, F. H. Arnold, G. Georgiou. ACS Synthetic Biology 1, 221-228 (2012). doi: 10.1021/sb300014t. |
| M. M. Y. Chen, C. D. Snow, C. L. Vizcarra, S. L. Mayo, F. H. Arnold. Protein Engineering, Design & Selection 25, 171-178 (2012). doi:10.1093/protein/gzs004 |
| M. M. Chen, P. S. Coelho, F. H. Arnold. Advanced Synthesis and Catalysis 354, 964-968 (2012). doi:10.1002/adsc.201100833 |
| S. Bastian, F. H. Arnold, in Microbial Metabolic Engineering, Methods and Protocols,” Q. Cheng (ed.). Methods in Molecular Biology, vol. 834, p. 17-31 (2012). Humana Press, N.Y. doi:10.1007/978-1-61779-483-4_2 |
| “Computational Thermostable Cellulase Engineering for Enhanced Biofuel Production” T. Lee, M. Farrow, F. H. Arnold. Protein Science 21 (S1), 71-72 (2012). doi:10.1002/pro.2113 |
| “High Resolution Models from Low Resolution Data: Recombination of Fragments by Crystallographic Structure Determination” C. Snow, E. Brustad, F. H. Arnold. Protein Science 21 (S1), 91-92 (2012). doi:10.1002/pro.2113 |
| “Metalloprotein-Based Contrast Sensors for Neurochemical Detection by MRI” V. Lelyveld, E. Brustad, F. H. Arnold. Protein Science 21 (S1), 184 (2012). doi:10.1002/pro.2113 |
2011 |
| T. M. Lee., M. F. Farrow, F. H. Arnold, S. L. Mayo. Protein Science 20, 1935-1940 (2011). doi:10.1002/pro.730 |
| M. F. Farrow, F. H. Arnold. Journal of Visualized Experiments, 54 (2011). http://www.jove.com/details.php?id=2942. doi:10.3791/2942 |
| F. H. Arnold. Microbe, ASM News 6, 316-318 (2011). Also hosted here. |
| A. Rentmeister, T. R. Brown, C. D. Snow, M. N. Carbone, F. H. Arnold. ChemCatChem 3, 1065-1071 (2011). doi:10.1002/cctc.201000452 |
| S. Bastian, X. Li, J. T. Meyerowitz, C. D. Snow, M. M. Y. Chen, F. H. Arnold.Metabolic Engineering 13, 345-352 (2011). doi:10.1016/j.ymben.2011.02.004 |
| “Cytochrome P450: Taming a Wild Type Enzyme” S. T. Jung, R. Lauchli, F. H. Arnold. Current Opinion in Biotechnology 22, 809-817. doi:10.1016/j.copbio.2011.02.008 |
| E. M. Brustad, F. H. Arnold. Current Opinion in ChemicalBiology 15, 201-210 (2011). doi: 10.1016/j.cbpa.2010.11.020 |
| J. C. Lewis, P. S. Coelho, F. H. Arnold. Chemical Society Reviews, 40, 2003-2021 (2011). doi: 10.1039/C0CS00067A |
| K. Brenner, F. H. Arnold. PLoS One 6(2): eprint 16791 (2011). doi:10.1371/journal.pone.0016791 |
| V. S. Lelyveld, E. Brustad, F. H. Arnold, A. Jasanoff. Journal of the American Chemical Society 133, 649-651 (2011). |
| F. Mingardon, J. D. Bagert, C. Maisonnier, D. L. Trudeau, F. H. Arnold. Applied and Environmental Microbiology 77(4), 1436-1442 (2011). |
2010 |
| R. Fasan, N. C. Crook, M. W. Peters, P. Meinhold, T. Buelter, M. Landwehr, P. C. Cirino, F. H. Arnold. Biotechnology and Bioengineering 108(3), 500-510 (2010). |
| M. Acar, B. F. Pando, F. H. Arnold, M. B. Elowitz, A. v. Oudernaarden. Science 329, 1656-1660 (2010). |
| J. C. Lewis, S. M. Mantovani, Y. Fu, C. D. Snow, R. S. Komor, C. H. Wong, F. H. Arnold. ChemBioChem 11(18), 2502-2505 (2010). doi: 10.1002/cbic.201000565 |
| P. Heinzelman, R. Komor, A. Kannan, P. Romero, X. Yu, S. Mohler, C. Snow, F. H. Arnold.Protein Engineering, Design and Selection 24,871-880 (2010). doi:10.1093/protein/gzq063 |
| C. Jackel, J. D. Bloom, P. Kast, F. H. Arnold, D. Hilvert. Journal of Molecular Biology 399, 541-546 (2010). |
| M. G. Shapiro, G. G. Westmeyer, P. A. Romero, J. O. Szablowski, B. Kuster, A. Shah, C. R. Otey, R. Langer, F. H. Arnold, A. Jasanoff. Nature Biotechnology 28 264-270 (2010). |
| M. Farrow, F. H. Arnold. Current Protocols in Protein Science (SUPPL. 61), pp. 26.2.1-26.2.20, 2010. |
2009 |
| F. H. Arnold. Cold Spring Harbor Symposia on Quantitative Biology, Volume LXXIV. 2009 Cold Spring Harbor Laboratory Press 978-087969870-6. |
| P. A. Romero, F. H. Arnold. Nature Reviews Molecular Cell Biology 10, 866-876 (2009). |
| M. J. Dougherty, F. H. Arnold. Current Opinion in Biotechnology, 20, 1-6 (2009). doi:10.1016/j.copbio.2009.08.005. |
| A. M. Sawayama, M. M. Chen, P. Kulanthaivel, M. S. Kuo, H. Hemmerle, F. H. Arnold. Chemistry, a European Journal 15, 11723-11729 (2009). doi:10.1002/chem.200900643 |
| P. Heinzelman, C. D. Snow, M. A. Smith, X. Yu, K. Arvind, K. Boulaware, A. Villalobos, S. Govindarajan, J. Minshull, F.H. Arnold. J. Biol. Chem. 284, 26229-26233 (2009). doi:10.1074/jbc.C109.034058. |
| J. C. Lewis, S. Bastian, C. S. Bennett, Y. Fu, Y. Mitsuda, M. M. Chen, W. A. Greenberg, C. H. Wong, F. H. Arnold. Proc. Natl. Acad. Sci. 106 (39), 16550-16555 (2009). doi/10.1073/pnas.0908954106. |
| J. C. Lewis, F. H. Arnold. Chimia 63, 309-312 (2009). doi:10.2533/chimia.2009.309. |
| J. D. Bloom, F. H. Arnold. Proc. Natl. Acad. Sci.106, 9995-10000 (2009). doi:10.1073/pnas.0901522106. |
| C. A. Tracewell, F. H. Arnold. Current Opinion in Chemical Biology13(1): 3-9 (2009). doi:10.1016/j.cbpa.2009.01.017. |
| P. Heinzelman, C. D. Snow, I. Wu, C. Nguyen, A. Villalobos, S. Govindarajan, J. Minshull, F. H. Arnold. Proc. Natl. Acad. Sci. 106, 5610-5615 (2009). doi10.1073pnas.0901417106. |
| D. J. Koch, M. M. Chen, J. B. van Beilen, F. H. Arnold, Applied and Environmental Microbiology 75(2), 337–344 (2009). |
| G. Saab-Rincon, Y. Li, M. Meyer, M. N. Carbone, M. Landwehr, F. H. Arnold. pp. 481-492 in Protein Engineering Handbook, S. Lutz & U.T. Bornscheuer (Eds.). |
| A. Rentmeister, F. H. Arnold, R. Fasan. Nature Chemical Biology 5(1): 26-28 (2009). in Nature. |
2008 |
| F. H. Arnold. Engineering & Science 2, 12-19 (2008). |
| K. Brenner, L. You, F. H. Arnold. Trends in Biotechnology 27, 4 (2008). |
| K. Brenner, L. You, F. H. Arnold. Trends in Biotechnology 26(9): 483-489 (2008). |
| R. Fasan, Y. T. Meharenna, C. D. Snow, T. L. Poulos, F. H. Arnold. Journal of Molecular Biology 383(5), 1069-1080 (2008) |
| F. K. Balagadde, H. Song, J. Ozaki, C. H. Collins, M. Barnet, F. H. Arnold, S. R. Quake, L. You. Molecular Systems Biology 4, 187 (2008). doi: 10.1038/msb.2008.24 |
| E. L. Haseltine, F. H. Arnold. Applied and Evironmental Microbiology 74, 437-445 (2008). . |
| F. K. Balagradde, H. Song, J. Ozaki, C. H. Collins, M. Barnet, F. H. Arnold, S. R. Quake, L. You Molecular Systems Biology 4, 187 (2008). |
2007 |
| K. Brenner, D. K. Karig, R. Weiss, F. H. Arnold. Proc. Natl. Acad. Sci. 104, 17300-17304 (2007). in Nature. |
| “Directed Evolution of Vibrio fischeri LuxR for Improved Response to Butanoyl-Homoserine Lactone” A. C. Hawkins, F. H. Arnold, R. Stuermer, B. Hauer, J. R. Leadbetter. Applied and Environmental Microbiology 73, 5775-5781 (2007). |
| Y. Li, D. A. Drummond, A. M. Sawayama, C. D. Snow, J. D. Bloom, F. Arnold. Nature Biotechnology 25, 1051-1056 (2007). |
| R. Fasan, M. M. Chen, N. C. Crook, F. H. Arnold. AngewChemie, Int. Ed. 46, 8414-8418 (2007). |
| J. D. Bloom, Z. Lu, D. Chen, A. Raval, O. S. Ventura, F. H. Arnold. BMC Biology 5, 29 (2007). |
| J. D. Bloom, F. H. Arnold, C. O. Wilke. Molecular Systems Biology 3, 76 (2007). |
| M. N. Carbone, F. H. Arnold. Current Opinion in Structural Biology 17, 454-549 (2007). |
| J. D. Bloom, P. A. Romero, Z. Lu, F. H. Arnold. Biology Direct 2, 17 (2007). |
| E. L. Haseltine, F. H. Arnold. Annual Review of Biophysics and Biomolecular Structure 36, 1-19 (2007). |
| M. Landwehr, M. Carbone, C. R. Otey, Y. Li, F. H. Arnold. Chemistry & Biology 14, 269-278 (2007). |
2006 |
| M. M. Meyer, L. Hochrein, F. H. Arnold, Protein Engineering, Design and Selection 19, 563-570 (2006). |
| J. D. Bloom, D. A. Drummond, F. H. Arnold, C. O. Wilke. Molecular Biology and Evolution 23(9), 1751-1761 (2006). |
| “Dual Selection Enhances the Signaling Specificity of a Variant of the Quorum-Sensing Transcriptional Activator LuxR” C. H. Collins, J. R. Leadbetter, F. H. Arnold. Nature Biotechnology 24, 708-712 (2006). Commentary in Nature Biotechnology. |
| M. Landwehr, L. Hochrein, C. R. Otey, A. Kasrayan, J. E. B�ckvall, F. H. Arnold. Journal of the American Chemical Society 128(18), 6058-6059 (2006). |
| C. R. Otey, M. Landwehr, J.B. Endelman, K. Hiraga, J. D. Bloom, F. H. Arnold. PLoS Biology 4(5), e112 (2006). in PLoS Biology; featured in Chemical & Engineering News. |
| “Engineering Cytochrome P450 BM3 for Terminal Alkane Hydroxylation” P. Meinhold, M. W. Peters, A. Hartwick, A. R. Hernandez, F. H. Arnold. Advanced Synthesis & Catalysis 348, 763-772 (2006). |
| F. H. Arnold. Nature Biotechnology 24(3), 328-330 (2006). |
| J. D. Bloom, S. T. Labthavikul, C. R. Otey, F. H. Arnold. Proc. Nat’l. Acad. Sci. USA 103(15), 5869-5874 (2006). |
| A. V. Tobias, F. H. Arnold. BBA-Molecular and Cell Biology of Lipids 1761(2), 235-246 (2006). |
| “Enantioselective Epoxidation of Terminal Alkenes to (R)- and (S)-Epoxides by Engineered Cytochromes P450 BM-3” T. Kubo, M. W. Peters, P. Meinhold, F. H. Arnold. Chemistry, a European Journal 12, 1216-1220 (2006). |
2005 |
| J. D. Bloom, M. M. Meyer, P. Meinhold, C. R. Otey, D. MacMillan, F. H. Arnold. Curr. Opin. Struct. Biol. 15, 447-452 (2005). |
| Y. Yokobayashi, F. H. Arnold. Natural Computing 4, 245-254 (2005). |
| C. R. Otey, G. Bandara, J. Lalonde, K. Takahashi, F. H. Arnold. Biotechnology and Bioengineering 93, 494-499 (2005). |
| D. A. Drummond, J. D. Bloom, C. Adami, C. O. Wilke, F. H. Arnold. Proc. Nat’l. Acad. Sci. USA 102(40), 14338-14343 (2005). Selected as Nature Research Highlight; commentary in Journal of Cell Biology. |
| P. Meinhold, M. W. Peters, M. M. Y. Chen, K. Takahashi, F. H. Arnold. ChemBioChem 6, 1765-1768 (2005). |
| F. K. Balagadd�, L. You, C. L. Hansen, F. H. Arnold, S. R. Quake. Science 309, 137-140 (2005). () |
| D. A. Drummond, B. L. Iverson, G. Georgiou, F. H. Arnold. Journal of Molecular Biology 350, 806-816 (2005). |
| A. K. Udit, N. Hindoyan, M. G. Hill, F. H. Arnold, H. B. Gray. Inorganic Chemistry 44(12), 4109-4111 (2005). |
| S. Basu, Y. Gerchman, C. H. Collins, F. H. Arnold, R. Weiss. Nature 434(7037), 1130-1134 (2005). |
| D. F. M�nzer, P. Meinhold, M. W. Peters, S. Feichtenhoferc, H. Griengla, F. H. Arnold, A. Glieder, A. de Raadt. Chem. Commun. 20, 2597-2599 (2005). |
| D. Umeno, A. V. Tobias, F. H. Arnold. Microbiology and Molecular Biology Reviews 69(1), 51-78 (2005). |
| D. A. Drummond, J. J. Silberg, M. M. Meyer, C. O. Wilke, F. H. Arnold. Proc. Natl. Acad. Sci. USA 102(15), 5380-5385 (2005). |
| C. H. Collins, F. H. Arnold, J. R. Leadbetter. Molecular Microbiology 55(3), 712-723 (2005). |
| J. D. Bloom, J. J. Silberg, C. O. Wilke, D. A. Drummond, C. Adami, F. H. Arnold. Proc. Natl. Acad. Sci. USA 102(3), 606-611 (2005). |
| A. Y. Fu, Y. Yokobayashi, F. H. Arnold, S. R. Quake. Chapter 5 in Flow Cytometry for Biotechnology, L. A. Sklar (Ed.), Oxford University Press, USA (2005). |
2004 |
| T. S. Wong, F. H. Arnold, U. Schwaneberg. Biotechnol. Bioengineering 85(3), 351-358 (2004). |
| K. Miyazaki, F. H. Arnold. pp. 43-60 in Phage Display: A Practical Approach, T. Clackson and H. Lowman (Eds.), Oxford University Press Inc., NY (2004). |
| J. J. Silberg, J. B. Endelman, F. H. Arnold, Methods in Enzymology 388, 35-42 (2004). |
| J. B. Endelman, J. J. Silberg, Z-G. Wang, F. H. Arnold. Protein Engineering, Design, & Selection 17(7), 589-594 (2004). |
| A. K. Udit, M. G. Hill, V. G. Bittner, F. H. Arnold, H. B. Gray. Journal of the American Chemical Society 126(33), 10218-10219 (2004). |
| A. K. Udit, F. H. Arnold, H. B. Gray. Journal of Inorganic Biochemistry 98(9), 1547-1550 (2004). |
| L. You, R. S. Cox III, R. Weiss, F. H. Arnold. Nature 428(6985), 868-871 (2004). |
| J. D. Bloom, C. O. Wilke, F. H. Arnold, C. Adami. Biophysical Journal 86, 2758-2764 (2004). |
| D. Umeno, F. H. Arnold. Journal of Bacteriology 186(5), 1531-1536 (2004). |
| C. R. Otey, J. J. Silberg, C. A. Voigt, J. B. Endelman, G. Bandara, F. H. Arnold. Chemistry and Biology 11, 309-318 (2004). |
| “Directing the Evolvable: Utilizing Robustness in Evolution” C. A. Voigt, S. L. Mayo, Z.-G. Wang, F. H. Arnold. pp. 105-134 in Robust Design: A Repertoire of Biological, Ecological and Engineering Case Studies,” E. Jen (Ed.), Oxford University Press (2004). |
| M. Alcalde, E. T. Farinas, F. H. Arnold. Journal of Biomolecular Screening 9(2), 141-146 (2004). |
| J. Malliaros, C. Quinn, F. H. Arnold, M. J. Pearse, D. P. Drne, T. J. Stewart, R. I. Macfarlan. Vaccine 22(29-30), 3968-3975 (2004). doi:10.1016/j.vaccine.2004.03.054 |
2003 |
| M. W. Peters, P. Meinhold, A. Glieder, F. H. Arnold. Journal of the American Chemical Society 125, 13442-13450 (2003). |
| O. Salazar, P. C. Cirino, F. H. Arnold. ChemBioChem 4, 891-893 (2003). |
| E. T. Farinas, M. Alcalde, F. H. Arnold, Tetrahedron 60, 525-528 (2003). |
| Two volumes of Directed Evolution Protocols edited by F. H. Arnold and G. Georgiou are now available from Humana PressMethods in Molecular Biology series (2003): Directed evolution library creation and Directed Enzyme Evolution. |
| D. Umeno, K. Hiraga, F. H. Arnold. Nucleic Acids Research 31(16), e91 (2003). |
| C. H. Collins, Y. Yokobayashi, D. Umeno, F. H. Arnold. Current Opinion in Biotechnology 14, 371-378 (2003). |
| K. Hiraga, F. H. Arnold. Journal of Molecular Biology 330, 287-296 (2003). |
| M. M. Meyer, J. J. Silberg, C. A. Voigt, J. B. Endelman, S. L. Mayo, Z-G. Wang, F. H. Arnold. Protein Science 12(8), 1686-1693 (2003). |
| P. C. Cirino, F. H. Arnold. Angewandte Chemie International Edition 42(28), 3299-3301 (2003). |
| P. C. Cirino, Y. Tang, K. Takahashi, D. A. Tirrell, F. H. Arnold. Biotechnology and Bioengineering 83, 729-734 (2003). |
| D. Umeno, F. H. Arnold, Applied and Environmental Microbiology 69, 3573-3579 (2003). , August 2003. |
| P. L. Wintrode, D. Zhang, N. Vaidehi. F. H. Arnold, W. A. Goddard III. Journal of Molecular Biology 327, 745-757 (2003). |
| T. Bulter, M. Alcalde, P. Meinhold, C. Schlachtbauer, F. H. Arnold. Applied and Environmental Microbiology 69, 987-995 (2003). |
| Y. Yokobayashi, C.H. Collins, J. R. Leadbetter, R.Weiss, F. H. Arnold. Advances in Complex Systems 6, 37-45 (2003). |
| F. H. Arnold, A. Glieder. Current Opinion in Biotechnology 14, 567-569 (2003). |
2002 |
| Y. Yokobayashi, R. Weiss, F. H. Arnold. Proc. Natl. Acad. Sci. USA 99, 16587-16591 (2002). |
| D. Umeno, A. V. Tobias, F. H. Arnold. Journal of Bacteriology 184, 6690-6699 (2002). |
| A. Glieder, E. T. Farinas, F. H. Arnold. Nature Biotechnology 20, 1135-1139 (2002). |
| P. C. Cirino, F. H. Arnold. Advanced Synthesis & Catalysis 344, 932-937 (2002). |
| L. H. Sun, T. Bulter, M. Alcalde, I. P. Petrounia, F. H. Arnold. ChemBiochem 8, 781-783 (2002). |
| A. Y. Fu, H-P. Chou, C. Spence, F. H. Arnold, S .R. Quake. Analytical Chemistry 74, 2451-2457 (2002). |
| C. A. Voigt, C. Martinez, Z-G. Wang, S. L. Mayo, F. H. Arnold. Nature Structural Biology 9(7), 553-558 (2002). |
| “Directed Protein Evolution” K. M. Mayer, F. H. Arnold. In Encyclopedia of Evolution, Vol. 1 (of 2), 268-271, M. Pagel (Ed.), Oxford University Press, NY, 2002 |
| “Staggered Extension Process (StEP) in vitro Recombination” A. M. Aguinaldo, F. Arnold. In Methods in Molecular Biology, Vol. 192, PCR Cloning Protocols, 2nd Edition, pp. 235-239 (B.-Y. Chan and H. W. Janes, Eds.), Humana Press Inc., Totowa, NJ, 2002. |
| “Exploring the Diversity of Heme Enzymes Through Directed Evolution” P. C. Cirino, F. H. Arnold. In Directed Molecular Evolution of Proteins, S. Brakmann and K. Johnsson (Eds.) Wiley-VCH, Germany, pp. 215-243, 2002. |
| “A Colorimetric Assay to Quantify Dehydrogenase Activity in Crude Cell Lysates” K. M. Mayer, F. H. Arnold. Journal of Biomolecular Screening 7(2), 135-140 (2002). |
| P. C. Cirino, F. H. Arnold. Current Opinion in Chemical Biology 6, 130-135 (2002). |
| J. M. Joern, P. Meinhold, F. H. Arnold. Journal of Molecular Biology 316, 643-656 (2002). |
2001 |
| E. T. Farinas, T. Bulter, F. H. Arnold. Current Opinion in Biotechnology 12, 545-551 (2001). |
| E. T. Farinas, U. Schwaneberg, A. Glieder, F. H. Arnold. Advanced Synthesis & Catalysis 343, 601-606 (2001). |
| “Dynamic Pattern Formation in a Vesicle-Generating Microfluidic Device” T. Thorsen, R. W. Roberts, F. H. Arnold, S. R. Quake. Physical Review Letters 86, 4163-4166 (2001). |
| T. Sakamoto, J. M. Joern, A. Arisawa, F. H. Arnold. Applied and Environmental Microbiology 67, 3882-3887 (2001). |
| V. Sieber, C. A. Martinez, F. H. Arnold, Nature Biotechnology19, 456-460 (2001). |
| “Patterns of Adaptation in a Laboratory Evolved Thermophilic Enzyme” P. L. Wintrode, K. Miyazaki, F. H. Arnold. Biochimica et Biophysica 1549, 1-8 (2001). |
| B. Morawski, S. Quan, F. H. Arnold. Biotechnology & Bioengineering 76, 99-107 (2001). |
| “Cost-Effective Whole-Cell Assay for Laboratory Evolution of Hydroxylases in E. coli” U. Schwaneberg, C. Otey, P. Cirino, E. Farinas, F. H. Arnold. Journal of Biomolecular Screening 6, 111-117 (2001). |
| F. H. Arnold, P. C. Wintrode, K. Miyazaki, A. Gershenson. Trends in Biochemical Sciences 26, 100-106 (2001). |
| L. Sun, I.P. Petrounia, M. Yagasaki, G. Bandara and F.H. Arnold, Protein Engineering 14: 699-704 (2001). |
| C.A. Voigt, S.L. Mayo, F.H. Arnold, and Z-G. Wang, Journal of Cellular Biochemistry 84(S37): 58-63 (2001). |
| “Staggered Extension Process (StEP) in vitro Recombination” A. M. Aguinaldo, F. H. Arnold. Chapter in PCR Cloning Protocols, Methods in Molecular Biology (2nd Edition) Eds. B. Chan and H. Janes, Humana Press (2001). |
| F. H. Arnold, Nature 409, 253-257 (2001). |
| “A Versatile High-Throughput Screen for Dioxygenase Activity Using Solid-Phase Digital Imaging” J. M. Joern, T. Sakamoto, A. Arisawa, F. H. Arnold. Journal of Biomolecular Screening 6, 219-223 (2001). |
A new volume (Vol 55, 2001) of Advances in Protein Chemistry entitled “Evolutionary Protein Design” has been published by Academic Press. This volume, edited by F.H. Arnold, contains excellent contributions from top evolutionary design laboratories: Babbitt, Benkovic, Stemmer, Georgiou, Stevens, Plueckthun, Barbas, Wang, and Arnold. ISBN:0120342553.
 |
| C. A.Voigt, S. L. Mayo, F. H. Arnold, Z-G. Wang. Proc. Natl. Acad. Sci. USA 98, 3778-3783 (2001). |
2000 |
| C. Schmidt-Dannert, D. Umeno, F. H. Arnold. Nature Biotechnology 18, 750-753 (2000). |
| B. Morawski, Z. Lin, P. Cirino, H. Joo, F. H. Arnold. Protein Engineering 13(5), 377-384 (2000). |
| “Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution” P. L. Wintrode, K. Miyazaki, F. H. Arnold. Journal of Biological Chemistry 275 (41), 31635-31640 (2000). |
| I. P. Petrounia, F. H. Arnold. Current Opinion in Biotechnology 11(4), 325-330 (2000). |
| K. Miyazaki, P. L. Wintrode, R. A. Grayling, D. N. Rubingh, F. H. Arnold. Journal of Molecular Biology 297, 1015-1026 (2000). |
| A. Gershenson, J.A. Schauerte, L. Giver, F. H. Arnold. Biochemistry 39, 4658-4665 (2000). |
| “Enzyme Stabilization by Directed Evolution” A. Gershenson, F. H. Arnold. Genetic Engineering Principles and Methods, Vol. 22, pp. 55-76, Plenum Press (2000). |
| O. May, P.T. Nguyen, F. H. Arnold. Nature Biotechnology 18, 317-320 (2000). |
| O. May, C. Voigt, F. H. Arnold. In Enzyme Catalysis in Organic Synthesis,” K. H. Drauz and H. Waldmann (Eds.), pp. 95-138, Vol. 1. Wiley-VCH (2000). |
| P. Wintrode, F. H. Arnold. pp. 161-226 in Advances in Protein Chemistry, Evolutionary Approaches to Protein Design,” F. H. Arnold (Ed.), Vol. 55. Academic Press (2000). |
| “Random Chimeragenesis by Heteroduplex Recombination” A. A.Volkov, Z. Shao, F. H. Arnold. Methods in Enzymology 328, 447-456 (2000). |
| “Methods for in vitro DNA Recombination and Random Chimeragenesis” A. A. Volkov, F. H. Arnold. Methods in Enzymology 328, 447-456 (2000). |
1999 |
| E. M. J. Gillam, A. M. A. Aguinaldo, L. M. Notley, D. Kim, R. G. Mundkowski, A. A. Volkov, F. H. Arnold, P. Soucek, J. J. DeVoss, F. P. Guengerich. Biochem. Biophys. Res. Comm. 265, 469-472 (1999). |
| A. Y. Fu, C. Spence, A. Scherer, F. H. Arnold, S. Quake. Nature Biotechnology 17, 1109-1111 (1999). |
| H. Joo, A. Arisawa, Z. Lin, F. H. Arnold. Chemistry & Biology 6(10), 699-706 (1999). |
| F. H. Arnold. Engineering & Science (Caltech), No. 1/2, pp. 40-50, 1999. |
| K. Miyazaki, F. H. Arnold. Journal of Molecular Evolution 49, 716-720 (1999). |
| J. Affholter, F. H. Arnold. CHEMTECH 29(9), 34-39 (1999). |
| A. Volkov, Z. Shao, F. H. Arnold. Nucleic Acids Research 27, e18 (1999). |
| B. Spiller, A. Gershenson, F. H. Arnold, R. C. Stevens. Proc. Natl. Acad. Sci. USA96, 12305-12310 (1999). |
| H. Joo, Z. Lin, F. H. Arnold. Nature 399, 670-673 (1999). |
| C. Schmidt-Dannert, F. H. Arnold. Trends in Biotechnology 17, 135-136 (1999). |
| S. W. Michnick, F. H. Arnold. Nature Biotechnology 17(12), 1159-60 (1999). |
| F. H. Arnold, A. A. Volkov. Current Opinion in Chemical Biology 3, 54-59 (1999). |
| “” F. H. Arnold, P. L. Wintrode. Chapter for the Encyclopedia of Bioprocess Technology, M. C. Flickinger and S. W. Drew (Eds.), Wiley, 1999. |
| “Directed Evolution of Mesophilic Enzymes Into Their Thermophilic Counterparts” F. H. Arnold, L. Giver, A. Gershenson, H. Zhao, K. Miyazaki. Annals of the New York Academy of Sciences 870, 400-403 (1999). |
| H. Zhao, F. H. Arnold, Protein Engineering 12, 47-53 (1999). |
| “Methods for Optimizing Industrial Enzymes by Directed Evolution” H. Zhao, J. C. Moore, A. A.Volkov, F. H. Arnold. In Manual of Industrial Microbiology and Biotechnology, 2nd Ed. A. L. Demain and J. E. Davies (Eds.) Chapter 49, pp. 597-604, ASM Press, Washington, DC, 1999. |
1998 |
| “When Blind is Better: Protein Design by Evolution” F. H. Arnold. Commentary in Nature Biotechnology 16, 617-618 (1998). |
| L. Giver, A. Gershenson, P. O. Freskgard, F. H. Arnold. Proc. Natl. Acad. Sci. USA 95, 12809-12813 (1998). |
| F. H. Arnold. Accounts of Chemical Research 31, 125-131 (1998). |
| L. Giver, F. H. Arnold. Current Opinion in Chemical Biology 2, 335-338 (1998). |
| F. H. Arnold, Commentary in Proc. Natl. Acad. Sci. USA 95, 2035-2036 (1998). |
| “Molecular Evolution by Staggered Extension Process (StEP) in vitro Recombination” H. Zhao, L. Giver, Z. Shao, J. A. Affholter, F. H. Arnold. Nature Biotechnology 16, 258-261 (1998). |
| Z. Shao, H. Zhao, L. Giver, F. H. Arnold. Nucleic Acids Research 26, 681-683 (1998). |
1997 |
| O. Kuchner, F. H. Arnold. Trends in Biotechnology 15, 523-530 (1997). |
| “Combinatorial Protein Design: Strategies for Screening Protein Libraries” H. Zhao, F. H. Arnold. Current Opinion in Structural Biology 7, 480-485 (1997). |
| J. C. Moore. H.-M. Jin, O. Kuchner, F. H. Arnold. Journal of Molecular Biology 272, 336-347 (1997). |
| H. Zhao, F. H. Arnold. Proc. Natl. Acad. Sci. USA 94, 7997-8000 (1997). |
| H. Zhao, F. H. Arnold. Nucleic Acids Research 25,1307-1308 (1997). |
| “Optimizing Industrial Enzymes by Directed Evolution” F. H. Arnold, J. C. Moore. Advances in Biochemical Engineering 58, 1-14 (1997). |
| “Langmuir Monolayer Characterization of Metal Chelating Lipids for Protein Targeting to Membranes” D. W. Pack, F. H. Arnold. Chemistry and Physics of Lipids 86(2), 135-152 (1997). |
1996 |
| “” Z. Shao, F. H. Arnold. Current Opinion in Structural Biology 6, 513-518 (1996). |
| F. H. Arnold. Chemical Engineering Science 51, 5091-5102 (1996). |
| “Strategy for the Directed Evolution of a Peptide Ligase” H. Zhao, L. You, F. H. Arnold. Proceedings of the 13th Enzyme Engineering Conference, Annals of the New York Academy of Sciences 799, 1-6 (1996). |
| “Directed Evolution of a para-Nitrobenzyl Esterase for Aqueous-Organic Solvents” J. Moore, F. H. Arnold. Nature Biotechnology 14, 458-467 (1996). |
| W. Frey, W. R. Schief, D. W. Pack, C. Chen, A. Chilkoti, P. Stayton, V. Vogel, F. H. Arnold. Proc. Natl. Acad. Sci. USA 93(10), 4937—4941 (1996). |
| “Directed Evolution of Subtilisin E in Bacillus subtilis to Enhance Total Activity in Aqueous Dimethylformamide” L. You and F.H. Arnold. Protein Engineering 9, 77-83 (1996). |
1994 |
| “Metal-Mediated Protein Stabilization” J.-H. Zhang and F.H. Arnold, Trends in Biotechnology 12: 189-192 (1994). |
1993 |
| K. Chen, F. H. Arnold. Proc. Natl. Acad. Sci. USA 90, 5618-5622 (1993). |
| “Protein Engineering for Unusual Environments” F. H. Arnold. Current Opinion in Biotechnology 4, 450-455 (1993). |
| “Engineering Proteins for Nonnatural Environments” F. H. Arnold. FASEB Journal 7, 744-749 (1993). |
| “Ruthenium-Mediated Protein Cross-Linking and Stabilization” A. Muheim, R .J. Todd, D. R. Casimiro, H. R. Gray, F. H. Arnold. Journal of the American Chemical Society 115, 5312-5313 (1993). |
1991 |
| “Enzyme Engineering for Nonaqueous Solvents. II. Additive Effects of Mutations on the Stability and Activity of Subtilisin E in Polar Organic Media” K. Q. Chen, A .C. Robinson, M. E. Van Dam, P. Martinez, C. Economou, F. H. Arnold. Biotechnology Progress 7(2), 125-129 (1991). |
| “Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media” K. Chen, F. H. Arnold. Biotechnology (NY) 9, 1073-1077 (1991). |
| “Protein Stabilization by Engineered Metal Chelation” J. Kellis, Jr., R. J. Todd, F. H. Arnold. Biotechnology (NY) 9, 994-995 (1991). |
| F. H. Arnold, B. L. Haymore. Science 252, 1796-1797 (1991). |
| “Cu(II)-Binding Properties of a Synthetic Metal-Binding Cytochrome c: His-X3-His in an a-Helix” R. Todd, M. Van Dam, D. Casimiro, B. L. Haymore, F. H. Arnold. Proteins: Struct. Funct. Genet. 10, 156-161 (1991). |
| “Characterization of His-X3-His Sites in alpha-Helices of Synthetic Metal-Binding Bovine Somatotropin” S. S. Suh, B. L. Haymore, F. H. Arnold. Protein Engineering 4, 301-305 (1991). |
1990 |
| “Engineering Enzymes for Non-Aqueous Solvents” F. H. Arnold. Trends in Biotechnology 8(9), 244-249 (1990). |
1988 |
| “Protein Design for Non-Aqueous Solvents” F. H. Arnold. Protein Engineering 2(1), 21-25 (1988). |